FastQCFastQC Report
Fri 8 Mar 2019
C04-CTCF-EVAN_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC04-CTCF-EVAN_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7673577
Sequences flagged as poor quality0
Sequence length35-80
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG498200.6492408950871281Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82410.1073945045446211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)