FastQCFastQC Report
Mon 19 Jun 2017
NA12878_PCRFRee_20170509.MPS12342217-E10.4212.1.pair2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNA12878_PCRFRee_20170509.MPS12342217-E10.4212.1.pair2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490121034
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG623550.013.844915145
CGCCGTA1293250.06.417603145
TCGCCGG615700.05.9452767145
CGTCGTG7184550.05.852658145
AGCGTCG7790700.05.8383093145
CGTGTAG6727600.05.748097145
GTGGTCG2372300.05.6679254145
TCGTGTA6653400.05.6128325145
GTCGCCG1942450.05.5713415145
TCGGTGG2865050.05.5710235145
GCCGTAT1414950.05.4916587145
GAGCGTC8607450.05.401375145
GCGTCGT7489100.05.3659062140-144
GTCGTGT7105250.05.354847145
TGGTCGC2235600.05.330373145
TCGCCGT1510900.05.2628436140-144
CTCGGTG3164400.05.218097145
GGTCGCC2323250.05.1729517145
AGAGCGG1653600.05.141826145
AAGAGCG10234050.05.1222496145
Produced by FastQC (version 0.11.5)