FastQCFastQC Report
Thu 20 Aug 2015
read2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenameread2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124291599
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG379850.014.5140412
ATATAGT449200.012.34187213
CGATTAG64300.011.75022745
TCATATA480850.011.54434811
GCGCTAA102100.011.52006142
TATAGTA499900.011.1860614
CGACCGT121750.010.9389351
CGGTAGG80300.010.31309960
GCCTATC537900.010.30145556
TCGCGTT95900.010.2871433
ATAGTAA567950.010.04052115
TAACAGG567600.010.0022140
TAGTAAA576100.09.8865316
ACCGTTG136100.09.8365063
TATATCG182700.09.6661135
TATTCGG287700.09.6056849
GTAAAAC582850.09.576529518
CGGCCTA360200.09.52242169
CACATGC620050.09.4263471
CGCTAAG127900.09.33241243
Produced by FastQC (version 0.11.3)